%0 Journal Article
%A Mengmeng CHENG
%A Bin GE
%A Beisen KOU
%A Wenbin GUAN
%A Hai LU
%A Huihong GUO
%A Hui LI
%T Identification and Expression Analysis of <i>HDAC</i> and <i>HAT</i> gene families in <i>Xanthoceras sorbifolium</i>
%D 2026
%R 10.7525/j.issn.1673-5102.2026.01.005
%J Bulletin of Botanical Research
%P 51-66
%V 46
%N 1
%X <p>Histone deacetylases（HDACs） and histone acetyltransferases（HATs） are a group of reversible enzymes that catalyze histone acetylation/deacetylation， playing crucial roles in plant growth and development. This study aimed to investigate the characteristics of histone deacetylase（XsHDAC） and histone acetyltransferase（XsHAT） family member genes and proteins in <i>Xanthoceras sorbifolium</i>， with a focus on their expression patterns during seed embryo development. Genome-wide identification of XsHDACs and XsHATs family members in <i>X. sorbifolium</i> was conducted. Bioinformatics analyses were performed to examine their physicochemical properties， gene structure， conserved motifs， chromosomal localization， synteny， and <i>cis</i>-acting elements. The expression patterns of <i>XsHDACs</i> and <i>XsHATs</i> genes<italic/>in different tissues and during seed embryo development were analyzed using quantitative real-time PCR. A total of 15 <i>XsHDACs</i> genes（belonging to the RPD3/HDA1， HD2， and SIR2 subfamilies） and eight <i>XsHATs</i> genes（belonging to the GNAT， MYST， CBP， and TAF<sub>II</sub>250 subfamilies） were identified from the <i>X. sorbifolium</i> genome. These genes were distributed across nine different chromosomes. Physicochemical analysis of XsHDACs and XsHATs family members revealed that most proteins in both families were hydrophilic and acidic. Subcellular localization predictions indicated that XsHDACs and XsHATs family proteins were primarily localized in the nucleus. Phylogenetic analysis showed a close evolutionary relationship between <i>X. sorbifolium</i> and <i>Litchi chinensis</i>， both belonging to the Sapindaceae family. Protein structure predictions demonstrated that XsHDACs and XsHATs family members were rich in random coils and <i>α</i>-helices. <i>Cis</i>-acting element analysis revealed that the promoters of <i>XsHDACs</i> and <i>XsHATs</i> genes<italic/>contain elements related to seed development， light response， hormone signaling， and stress responses. Quantitative real-time PCR revealed that most <i>XsHDACs</i> and <i>XsHATs</i> members were highly expressed in the embryo of seed， with increased expression levels during late-stage embryogenesis. The <i>XsHDACs</i> and <i>XsHATs</i> gene families exhibited both evolutionary conservation and specificity. The expression results suggested their potential crucial roles in the embryonic development and oil biosynthesis in <i>X. sorbifolium.</i></p>
%U https://bbr.nefu.edu.cn/EN/10.7525/j.issn.1673-5102.2026.01.005