%0 Journal Article
%A Jingjing ZHANG
%A Haixia LUO
%A Yanping HONG
%A Shicheng ZHAO
%T Optimization of <i>Agrobacterium rhizogenes</i>-Mediated Genetic Transformation System of <i>Solanum nigrum</i>
%D 2026
%R 10.7525/j.issn.1673-5102.2026.01.010
%J Bulletin of Botanical Research
%P 111-119
%V 46
%N 1
%X <p>The aim of this study was to establish an <i>Agrobacterium rhizogenes</i>-mediated genetic transformation system for <i>Solanum</i><i>nigrum</i>（black nightshade） with the goal of enhancing transformation efficiency and increasing its total flavonoid content.<italic/>Based on this system， the recombinant plasmid PBI121-<i>6flag</i>-<i>SnCHI</i> was constructed， the hairy roots of <i>SnCHI</i> gene transformation were induced by <i>Agrobacterium</i>-mediated pathway， and the content of total flavonoids in the hairy roots of <i>S.nigrum</i> after <i>SnCHI</i> gene transformation was investigated. The results showed that the induction rate of hairy roots of <i>S. nigrum</i> could reach 88.49% under the conditions of OD<sub>600</sub> value of 1.0， infection for 25 min and co-culture for 24 h. The qRT-PCR detection of transgenic <i>SnCHI</i> hairy roots showed that the gene expression level was 28.81 times that of the blank group. The total flavonoid content of <i>SnCHI</i> transgenic hairy roots was determined， and it was found that the total flavonoid content of <i>SnCHI</i> transgenic hairy roots was up to 4.14 times that of the blank group. The genetic transformation system of <i>S. nigrum</i> was successfully established， and the function of <i>SnCHI</i> gene was studied， which provided a reference for tissue culture and genetic transformation of the same genus.</p>
%U https://bbr.nefu.edu.cn/EN/10.7525/j.issn.1673-5102.2026.01.010