%0 Journal Article
%A Min CHEN
%A Meng-Fei TIAN
%A Chang-Yin FENG
%A Xian-Ming MENG
%A Meng LUO
%A Fan-Song MU
%T Simultaneous Determination of Baschnaloside and Rossicaside B in <i>Boschniakia rossica</i> by HPLC
%D 2021
%R 10.7525/j.issn.1673-5102.2021.03.014
%J Bulletin of Botanical Research
%P 436-440
%V 41
%N 3
%X <p>In order to establish an HPLC method for the simultaneous determination of baschnaloside and rossicaside B in <i>Boschniakia rossica</i> dry whole， a chromatographic column： Klimail 100-5 C<sub>18</sub> column（250 mm×4.6 mm， 5 μm）； mobile phase： acetonitrile as mobile phase A， 0.5% formic acid aqueous solution as mobile phase B， gradient elution conditions： 0?12 min， 15% A； 12?30 min， 15%?20% A； 30?40 min， 20%?25% A； 40?45 min， 25%?30% A； 45?60 min， 30%?100% A； flow rate： 1.0 mL·min<sup>-1</sup>； detection wavelength： 260 nm. Column temperature： 30℃. Injection volume： 20 μL. The linear range of naringin of baschnaloside was 4.688?150 μg·g<sup>-1</sup>（<i>R</i><sup>2</sup>=0.999 5）； the linear range of rossicaside B was 3.438?110 μg·g<sup>-1</sup>（<i>R</i><sup>2</sup>=0.999 1）； the average recoveries were 97.86% and 96.55%， and RSDs were 1.01 and 1.23（n=9）， respectively. A high-performance liquid chromatography method was developed for the simultaneous determination of two components， baschnaloside and rossicaside B in <i>B.rossica</i>. The methodological verification results show that the method is simple， fast， and reproducible， and can be used to determine the contents of baschnaloside and rossicaside B in<i> B.rossica</i>.</p>
%U https://bbr.nefu.edu.cn/EN/10.7525/j.issn.1673-5102.2021.03.014